Effects of Microbubble Size on Ultrasound-Mediated Gene Transfection in Auditory Cells

Figure 7

Transfection results for HEI-OC1 cells with albumin/dextrose MBs of three different sizes. (a, a′) Fluorescence and bright-field images of living cells in the groups of 1.39 μm albumin/dextrose MBs combined with plasmid, (b, b′) US combined with 1.39 μm albumin/dextrose MBs and plasmid, (c, c′) 2.12 μm albumin/dextrose MBs combined with plasmid, (d, d′) US combined with 2.12 μm albumin/dextrose MBs and plasmid, (e, e′) 3.47 μm albumin/dextrose MBs combined with plasmid, and (f, f′) US combined with 3.47 μm albumin/dextrose MBs and plasmid. (g) Fluorescence intensities for MBs of three sizes quantified and normalized relative to the control group. Scale bar = 200 μm. Results are expressed as mean ± standard deviation with for each bar. indicates .