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BioMed Research International
Volume 2014, Article ID 840921, 8 pages
Research Article

Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients

1Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy
2Department of Biology, University of Padova, Via 8 Febbraio 2, 35122 Padova, Italy
3Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy
4Department of Public Health, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy
5Department of Medicine, University of Salerno, Via Salvatore Allende 43, 84084 Baronissi, Italy

Received 27 February 2014; Revised 13 May 2014; Accepted 27 May 2014; Published 10 July 2014

Academic Editor: Kostas Iatrou

Copyright © 2014 Giuseppina Della Vittoria Scarpati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.