-Shogaol Inhibits α-MSH-Induced Melanogenesis through the Acceleration of ERK and PI3K/Akt-Mediated MITF Degradation
Figure 3
-Shogaol inhibits melanogenic regulatory protein expression in B16F10 cells. (a) The tyrosinase, TRP-1, TRP-2, and MITF levels were determined by western blotting and β-actin was used as loading control. Relative expression levels were compared to α-MSH-treated cells and presented as the mean ± SD for three independent experiments performed in triplicate. (b) Total RNA from -shogaol stimulated B16F10 cells at the indicated time was collected. MITF mRNA levels were examined by real-time RT-PCR using GAPDH as an internal control ( versus control).
