[6]-Shogaol Inhibits α-MSH-Induced Melanogenesis through the Acceleration of ERK and PI3K/Akt-Mediated MITF Degradation

Table 1

Effect of [6]-shogaol on α-MSH-induced tyrosinase activity and melanin synthesis in B16F10 cells.

	Tyrosinase activity (% of 𝛼-MSH control)
[6]-Shogaol (𝜇M)	1	5	10	15	20	Arbutin (2 mM)
	87.91^a	68.19^b	58.03^b	51.99^b	43.68^b	64.26^b
	Melanin content (% of 𝛼-MSH control)
Inhibitors 20 𝜇M/ [6]-shogaol 20 𝜇M	[6]-Shogaol	U0126 [6]-shogaol		PD98059  [6]-shogaol	LY294002  [6]-shogaol	Arbutin (2 mM)
	72.13^b	95.21^b		97.43^b	102.05^b	66.06^b

^a𝑃<0.05; ^b𝑃<0.001.