Construction and Characterization of a Bacterial Artificial Chromosome Library for the Hexaploid Wheat Line 92R137
Figure 1
Partial digestion of DNA in half plugs. Lanes 1–8 contain DNA samples digested with restriction enzyme at the increasingly higher concentrations. (a) Partial digestions of half DNA plugs with serial dilutions of HindIII at 37°C for 30 min. (b) Partial digestions of half DNA plugs with serial dilutions of BamHI at 37°C for 30 min. Plug pieces were separated on 1% agarose gel in 0.5x TBE and run in the CHEF DR-III System (Bio-Rad) at 6 V/cm, 1–50 s switch time, linear ramp, 120° angle at 14°C for 18 h. M: the λ PFG marker.
