BioMed Research International / 2014 / Article / Fig 3

Research Article

Inhibition of Hydrogen Sulfide Production by Gene Silencing Attenuates Inflammatory Activity by Downregulation of NF-κB and MAP Kinase Activity in LPS-Activated RAW 264.7 Cells

Figure 3

The effect of siRNA on the phosphorylation of ERK1/2 in RAW 264.7 cells macrophages. Cells were incubated with 100 ng/μL LPS for 30 min and cell lysates were examined by western blot using anti-ERK1/2 and phosphor-ERK1/2 (P-ERK) antibodies. (a) Results shown are representative blots from three independent experiments. (b and c) The histograms represent the ratios of the optical density of phosphor-ERK1/2: total ERK1/2 expressed as fold control. Data shown are the mean ± SD of the three experiments.

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