Atg6/UVRAG/Vps34-Containing Lipid Kinase Complex Is Required for Receptor Downregulation through Endolysosomal Degradation and Epithelial Polarity during Drosophila Wing Development
Figure 3
Electron microscopy of pupal wings. (a) Ultrastructure of control cells. (b) Depletion of Atg6 by RNAi resulted in the massive accumulation of enlarged multivesicular bodies (asterisks), dense multivesicular-body like structures (arrows), and multilamellar bodies (open arrows). (c) All of these structures could be found in Atg6 mutant pupal wings. (d) The depletion of Atg8a did not alter the ultrastructure of pupal wing cells. (e) Similar to Atg6, UVRAG RNAi also resulted in the accumulation of aberrant endolysosome-like structures (MVBs: arrows, MLBs: open arrows). (f) The depletion of Atg14 did not alter the ultrastructure of pupal wing cells. M: mitochondria, L: lipid droplet, and Ly: lysosome. For genotypes, see Table S4. Scale bars represent 1 μm.
