Comparison of Quasispecies Diversity of HCV between Chronic Hepatitis C and Hepatocellular Carcinoma by Ultradeep Pyrosequencing

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Region</td><td align="center" colspan="2">          Primer name</td><td align="left">Primer sequence (<svg height="17.9125" id="M2" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 53.25 17.9125" width="53.25" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,17.65)"><path d="M153 550l-26 -186q79 31 111 31q90 0 141.5 -51t51.5 -119q0 -93 -89 -166q-85 -69 -173 -71q-32 0 -61.5 11.5t-41.5 23.5q-18 17 -17 34q2 16 22 33q14 9 26 -1q61 -50 124 -50q60 0 93 43.5t33 104.5q0 69 -41.5 110t-121.5 41q-53 0 -102 -20l38 305h286l6 -8
l-26 -65h-233z" id="x35"></path></g>
<g transform="matrix(.012,-0,0,-.012,8.225,9.488)"><path d="M227 744l-123 -338l-31 15l73 368q12 3 41.5 -8t36.5 -20z" id="x2032"></path></g>
<g transform="matrix(.017,-0,0,-.017,19.938,17.65)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g><g transform="matrix(.017,-0,0,-.017,40.863,17.65)"><path d="M285 378v-2q65 -13 102 -54.5t37 -97.5q0 -57 -30.5 -104.5t-74 -75t-85.5 -42t-72 -14.5q-31 0 -59.5 11t-40.5 23q-19 18 -16 36q1 16 23 33q13 10 24 0q58 -51 124 -51q55 0 88 40t33 112q0 64 -39 96.5t-88 32.5q-29 0 -64 -11l-6 29q77 25 118 57.5t41 84.5
q0 45 -26.5 69.5t-68.5 24.5q-67 0 -120 -79l-20 20l43 63q51 56 127 56h1q66 0 107 -37t41 -95q0 -42 -31 -71q-22 -23 -68 -54z" id="x33"></path></g>
<g transform="matrix(.012,-0,0,-.012,49.037,9.488)"><path d="M227 744l-123 -338l-31 15l73 368q12 3 41.5 -8t36.5 -20z" id="x2032"></path></g>
</svg>)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left" rowspan="4">Core</td><td align="center" rowspan="2">1st</td><td align="center">B1xL.1-AP1</td><td align="left"> GGGCGACACTCCACCATAG</td></tr><tr><td align="center">B1xR.1-AP1</td><td align="left">  GCCGGCRAARAGYAGCAYC</td></tr><tr><td align="center" rowspan="2">2nd</td><td align="center">B1xL.1-AP2</td><td align="left">  ACTCCCCTGTGAGGAACTAY</td></tr><tr><td align="center">B1xR.1-AP3</td><td align="left">TGGGATCCGGAGYARCTG</td></tr><tr><td align="center" colspan="4"><hr/></td></tr><tr><td align="left" rowspan="4">E1/E2</td><td align="center" rowspan="2">1st</td><td align="center">B1yL.1-AP1</td><td align="left">TTCTGYTCCGCYATGTAYG</td></tr><tr><td align="center">B1yR.1-AP1</td><td align="left">  CACAAGRAAGGAGAGRANRC</td></tr><tr><td align="center" rowspan="2">2nd</td><td align="center">B1yL.1-AP3</td><td align="left"> GAACTGGTCRCCYACARCRG</td></tr><tr><td align="center">B1yR.1-AP2</td><td align="left"> GGACCACCARGTTCTCYARG</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Primer sequences for PCR amplification of HCV genome encoding structural proteins.

BioMed Research International

tab1

Table 1

Table 1: Comparison of Quasispecies Diversity of HCV between Chronic Hepatitis C and Hepatocellular Carcinoma by Ultradeep Pyrosequencing 