BioMed Research International / 2014 / Article / Fig 1

Research Article

A RXR Ligand 6-OH-11-O-Hydroxyphenanthrene with Antitumour Properties Enhances (−)-Epigallocatechin-3-gallate Activity in Three Human Breast Carcinoma Cell Lines

Figure 1

Cytotoxicity assay and apoptosis after EGCG, IIF, and EGCG + IIF treatments. (a) SRB assay after 72 h treatment. Cytotoxicity was found to increase after both individual and combined (EGCG + IIF) treatments. CTRL: untreated cells. Data shown represent the % treated on control samples of three replicas confirmed in two independent experiments. (b) DAPI staining. Apoptotic nuclei were detected by DAPI staining after 72 h incubation with EGCG, IIF, and EGCG + IIF at the indicated concentrations. The number of apoptotic nuclei for field was recorded by epifluorescence microscope and the average percentage was calculated. (c) Apoptosis Bax and Bcl2 detection in control (CTRL) and treated samples after 24 h incubation with EGCG, IIF, and EGCG + IIF at the indicated concentrations. Significant increase in Bax expression and decrease in Bcl2 expression were detected. ; . n.d.: not detected as significant.
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