BioMed Research International / 2014 / Article / Fig 2

Research Article

A RXR Ligand 6-OH-11-O-Hydroxyphenanthrene with Antitumour Properties Enhances (−)-Epigallocatechin-3-gallate Activity in Three Human Breast Carcinoma Cell Lines

Figure 2

LR67 qRT-PCR and LR67 protein expression. (a) LR67 qRT-PCR: a highly significant increase in LR67 mRNA expression was detected in MDA-MB-231 and MCF-7TAM cells, but not in MCF-7 cells. CTRL: untreated cells. (b) Western blot analysis of LR67: EGCG + IIF treatment was effective in increasing LR67 expression significantly in MCF-7 and MDA-MB-231 cells, whereas a trend was only detected in MCF-7TAM cells. (c) Western blot analysis of LR67 in MDA-MB-231 cells after transfection with vehicle (plV) and pLR67: the significant increase in LR67 protein expression found in MDA-MB-231 cells transfected with pLR67 did not parallel a comparable cell viability decrease (d) in plV and pLR67 transfected MDA-MB-231 cells (SRB assay). ; ; . n.d.: not detected as significant.
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853086.fig.002b
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