[Retracted] Exogenous IL-4-Expressing Bone Marrow Mesenchymal Stem Cells for the Treatment of Autoimmune Sensorineural Hearing Loss in a Guinea Pig Model
Figure 2
Validation of lentivirus pll3.7-IL-4. (a) Diagram of lentivirus pll3.7-IL-4. (b) Recombinant lentivirus pll3.7-IL-4 DNA digested with HindIII enzyme (left lane) compared with molecular marker bands (right lane). Gene name: rat IL-4 CDS-X2746G; clone ID#: D6466-2 RES:HindIII. Primers used for PCR amplification of IL-4 were 5′-GCTATTGATGGGTCTCACCC-3′ (F) and 5′-CAGGACGTCAAGGTACAGGA-3′ (R). The expected length of the PCR product was 412 bp and the annealing temperature used in PCR reactions was 60°C.
