Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
Figure 4
3D image of E18.5 epidermis whole-mount, follicle measurements (Keyence VHX-1000). ((a), (b)) Control C57Bl6 epidermis. ((c), (d)) Mutant epidermis. ((a), (c)) Early stage follicles. ((b), (d)) Medium stage follicles. (e) Individual height values of primordial follicles, early stages (up to 4 μm) are outlined with the box. Height values of the individual hair follicles were sequentially distributed in increasing order. (f) Average width at the base of hair follicles. Results are presented as mean ± SEM with the level of significance . Mutant follicles are significantly wider than control ones, as indicated by the asterisk. ((a), (b), (c), (d)) Earlier stage: ES. Basal layer: BL. Medium stage: MS. The distance between pink lines A and B corresponds to the height and the distance between blue lines C and D corresponds to the width. White square is the cross section of the hair follicle. Red point is the beginning of the follicle section; white point indicates the completion of the section.
