Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species

Table 1

Oligonucleotide primers and adapters. (a) Oligonucleotides used to find the cDNAs corresponding to tags. (b) Oligonucleotides used in real-time PCR.

(a)  

Name of the oligonucleotides	Oligonucleotides sequence (5′-3′)	Annealing temperature (°C)
External primer T7	CTAATACGACTCACTATAGGGC	66
Internal primer NotRsa	AGCGTGGTCGCGGCCGAGGT	68
GAPDH for.; GAPDH rev.	GTGGACGGCCCCTCTGC; TCTGGTGGGCACCACGG	63
Suppression adapter (equimolar mixture of preliminary annealed oligonucleotides T7NotRsa and Rsa_10)	T7NotRsa; CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT; Rsa_10; ACCTGCCCGG
Tag no. 89	GTACTTTAATGGATGATCTCC	56
Tag no. 81	GTACAAAAAGACTGCTGTTCC	60

(b)  

Name of the oligonucleotides	Oligonucleotides sequence (5′-3′)	Annealing temperature (°C)
GAPDH for.; GAPDH rev.	GTGGACGGCCCCTCTGC; TCTGGTGGGCACCACGG	63
1ep for.; 1ep rev.	TGGTGTAGGTCTCCTGGAC; CTCCACCTATGAGGACCAG	63
2net for.; 2net rev.	CAGCTCCTCCAGACGCACC; CGCTGGAGCTGTTCGACAAC	63
3st for.; 3st rev.	TGGAGGAGAAGATAGTGGACTTGT; AGTAATCTGACTTTGTTGGTGAACT	63
4fgf for.; 4fgf rev.	CGGTACAGCGTCGATGAGTAG; CACCATAAATGGGACCAAGG	63
5tnf for.; 5tnf rev.	CATTGCAGTCCTAGTCTCTCT; AGTGGTATCAACGCAGAGTAC	63
6tr for.; 6tr rev.	CAGGACAATGACCCAACACAC; TGAGGTCGGGGGATTGTG	63
7deh for.; 7deh rev.	GAGGAGGTATTTAAAGAATCGG; CCTCAGCTTTAAATTTGACCAC	63
8atp for.; 8atp rev.	GGCAGGTGAACTCCACAATC; CAGGAGTGCTGGAATCAAGG	63