Table 2: Summary of esterase B1 purification from Bacillus subtilis C5.

Purification stepTotal activity (U)Total protein (ug)Specific activity (U/mg)Yield (%)Purification fold

Culture supernatant75.52216.234.071001.0
(NH4)2SO4 precipitation2121160.85182.6256.165.36
Sephadex G-10035.563.75556.869.416.34