Role of Calcium Signaling in the Transcriptional Regulation of the Apicoplast Genome of Plasmodium falciparum

Figure 3

Identification of an apicoplast targeted protein with EF-hand domain. (a) Immunofluorescence microscopy done (anti-GFP) on parasites episomally expressing MAL13P1.156-GFP fusion shows typical apicoplast pattern. (b) Antibody against the c-terminal GFP detects MAL13P1.156 full-length protein with an apicoplast targeted protein signature (T-transfected, C-Control). (c, d) Colocalisation with Mitotracker-Red and apicoplast encoded tuF, respectively. (e) MAL13P1.156 multiple alignments with the structural homologues obtained from a sequence blast on PDB. EhCBP-Entamoeba histolytica calcium binding protein, CpCDPK3-Cryptosporidium parvum calcium-dependent protein kinase3, NCS1p-calcium binding protein NCS-1 Schizosaccharomyces pombe, and TgCDPK1-Toxoplasma gondii calcium dependent kinase1.