The Sirtuin 3 Expression Profile Is Associated with Pathological and Clinical Outcomes in Colon Cancer Patients
Figure 3
SIRT3 mRNA and protein expressions in SW480 and DLD-1 cells transfected with SIRT3 siRNA compared with cells transfected with control siRNA. (a) Real-time PCR quantitative analyses show that the SIRT3 mRNA expression levels were dramatically decreased after the SIRT3 siRNA transfection in both SW4980 and DLD-1 cell lines. (b) shows the typical western bolt bands of SIRT3, HIF1α, and VEGF in SW480 and DLD-1 cell lines. Quantitative analyses of relative expression ratios of SIRT3 were significantly inhibited by siRNA transfection compared to control siRNA (†). Also we analyzed the HIF-1α expression level after SIRT3 siRNA transfection. We found that HIF1 was markedly increased in cells with low SIRT3 expression. We next analyzed the angiogenesis regulator VEGF-A expression level. The SIRT3 inhibition unregulated the VEGF-A expression levels in cultured cells.
