Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes
Table 1
Effects of verbascoside on the in vitro meiotic maturation of prepubertal lamb oocytes.
Verbascoside concentration (M)
Number of cultured oocytes
IVM medium type
Nuclear chromatin configuration number (%)
GV
MI to TI
MII + PB
Degenerated
0
93
1
9 (9.7)c
6 (6.5)
59 (63.4)
19 (20.4)a
111
2
27 (24.3)d
17 (15.3)
60 (54.1)
7 (6.3)b
204
(*)
36 (17.6)
23 (11.3)
119 (58.3)
26 (12.7)
1.03
79
1
11 (13.9)
12 (15.2)
44 (55.7)
12 (15.2)
118
2
27 (22.9)
21 (17.8)
61 (51.7)
9 (7.6)
197
(*)
38 (19.3)
33 (16.8)
105 (53.3)
21 (10.7)
2.06
98
1
12 (12.2)c
6 (6.1)a
67 (68.4)c
13 (13.3)
116
2
33 (28.4)d
21 (18.1)b
50 (43.1)d
12 (10.3)
214
(*)
45 (21)
27 (12.6)
117 (54.7)
25 (11.7)
4.11
98
1
11 (11.2)c
13 (13.3)
58 (59.2)
16 (16.3)
137
2
36 (26.3)d
23 (16.8)
63 (46)
15 (10.9)
235
(*)
47 (20)
36 (15.3)
121 (51.5)
31 (13.2)
GV: germinal vesicle, M: metaphase, T: telophase, and PB: polar body. *Pooled data of oocytes cultured in medium 1 + medium 2. Chi-square test and multifactor ANOVA test: within each medium type and pooled data, VB-treated versus controls: NS; between media, within each VB concentration: ; .
