Phytochemicals: A Multitargeted Approach to Gynecologic Cancer Therapy
Figure 3
Hirsutenone-facilitated CDDP-induced apoptosis in chemoresistant OVCA cells is mediated by AIF and by suppressed XIAP-AIF interaction. (a) Effects of hirsutenone (10 μM) and CDDP (10 μM) treatment (12 h) on AIF nuclear translocation, in the presence and absence of XIAP overexpression. Chemoresistant cells were transfected with XIAP overexpression constructs (Pc-XIAP, 0.1 μg, 48 h) or GFP control constructs (Pc-GFP, 0.1 μg, 48 h), prior to treatment. Blue: DAPI, red: AIF, and green: TOM20 (mitochondrial membrane marker). (b) Effects of XIAP overexpression on apoptosis induced by hirsutenone (10 μM) and CDDP treatment (10 μM, 24 h). Chemoresistant cells were transfected with XIAP overexpression constructs (Pc-XIAP, 1 μg, 48 h) or GFP control constructs (Pc-GFP, 1 μg, 48 h), prior to treatment. (c) Quantification of AIF nuclear localization data shown in (a). Nuclear signal was quantified using Image J software (; versus respective DMSO control (CTL)). Taken from [46].
