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BioMed Research International
Volume 2014 (2014), Article ID 891402, 11 pages
http://dx.doi.org/10.1155/2014/891402
Research Article

Variations in KIR Genes: A Study in HIV-1 Serodiscordant Couples

1Department of Infectious Diseases Biology, National Institute for Research in Reproductive Health (DHR/ICMR), J. M. Street, Parel, Mumbai 400012, Maharashtra, India
2Department of Microbiology, Seth G. S. Medical College & K.E.M Hospital, J. M. Street, Parel, Mumbai 400012, Maharashtra, India

Received 21 February 2014; Revised 3 April 2014; Accepted 3 April 2014; Published 27 April 2014

Academic Editor: Lucia Lopalco

Copyright © 2014 Vijay R. Chavan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. NK cells have anti-HIV activity mediated through killer cell immunoglobulin-like receptors (KIRs). The current prospective cohort study evaluated whether variation in KIR genes is associated with HIV infection in discordant couples (DCs), where one spouse remains seronegative (HSN) despite repeated exposure to the HIV. Methods. KIR was genotyped using PCR SSP. Viral load and CD4 counts were estimated using commercially available reagents. Data were analyzed using SPSS software. Results. Among the 47 DCs, HSN spouses had significantly ( ) higher frequencies of KIR3DS1. Regression analysis revealed significant ( ) association of KIR2DS1 with low viral load. KIR2DS4 variant was associated ( ) with high viral load. Three pairs of KIR genes were in strong LD in HSNs and two pairs in HSPs. There were 60 KIR genotypes, and 16 are reported the first time in the Indian population. Exclusive genotypes were present either in HSPs ( , 11 unique genotypes) or in HSNs ( , 9 unique genotypes). Conclusions. This study highlights for the first time in the Indian population an association of KIR genes in HIV infection where presence of exclusive and unique genotypes indicates possible association with either HIV infection or with protection.