Tumorigenic Factor CRIPTO-1 Is Immunolocalized in Extravillous Cytotrophoblast in Placenta Creta
Expression of CRIPTO-1 and cell markers in healthy placentas. (A) Representative histological sections demonstrating the immunolocalization of vimentin (Vm: a, d), cytokeratin (CK: b, e), and CRIPTO-1 (CR-1: c, f) in placental beds from healthy placentas at 36 (a–c) and 38 (d–f) weeks of gestation. Arrowheads indicate cells reactive to cytokeratin and CRIPTO-1 in semiserial histological sections. Asterisks identify vascular areas stained for vimentin and CRIPTO-1 (a, c), but not for cytokeratin (b). (g–i) Negative control of the immunohistochemistry reactions in which the respective primary antibody has been omitted. Immunoperoxidase, Mayer’s hematoxylin counterstaining. Bar in (a) = 200 μm in all figures.