Trichinella spiralis Excretory-Secretory Products Protect against Polymicrobial Sepsis by Suppressing MyD88 via Mannose Receptor
Figure 4
TsES suppressed MyD88/NF-κB signaling via MR on peritoneal macrophages from septic patients. Macrophages from peritoneal lavage or abdominal cavity flushing fluid were collected from septic patients and the control group. Peritoneal macrophages were treated with TsES (5 μg/mL) for 6 h. Membrane protein fractions were extracted. MR expression was detected by western blot. compared to the control group. compared to the sepsis group (a). Peritoneal macrophages from septic patients were treated with a specific blocking antibody to MR (5 μg/mL) for 2 h and then with TsES for 6 h. Supernatant TNF-α, IL-1β, IL-10, and TGFβ1 levels were detected by ELISA (b). MyD88 expression in macrophages was detected by western blot (c). NF-κB activity was determined using the NF-κB p50/p65 Transcription Factor Assay Kit (d). Data are mean ± SD of three independent experiments, analyzed in triplicate. with respect to macrophages in medium alone. with respect to macrophages stimulated with TsES.
