BioMed Research International

Research Article

Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages

Table 1


	siRNA sequence	siRNAs	Tm °C

a	AS 3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 1 wt	76	—
b	SS 5′CGUACGCGGAAUACUUCGATT3′
C	3′TTGCAUGCGCCUUAUGAAGCT^NHCOt^OH5′	siRNA 2	65	−11
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 3	65	−11
D	t^CONHTCGUACGCGGAAUACUUCGATT3′
C	3′TTGCAUGCGCCUUAUGAAGCT^NHCOt^OH5′	siRNA 4	65	−11
D	t^CONHTCGUACGCGGAAUACUUCGATT3′
E	3′ttGCAUGCGCCUUAUGAAGCT^NHCOt^OH5′	siRNA 5	66	−10
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 6	66	−10
F	t^CONHTCGUACGCGGAAUACUUCGAtt3′
E	3′ttGCAUGCGCCUUAUGAAGCT^NHCOt^OH5′	siRNA 7	66	−10
F	t^CONHTCGUACGCGGAAUACUUCGAtt3′
G	3′TTGCAUGCGCCUUAUGAAGCT^NHCOt^OP5′	siRNA 8	67	−9
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 9	66	−10
H	t^CONHTCGUACGCGGAAUACUUCGATT3′
G	3′TTGCAUGCGCCUUAUGAAGCT^NHCOt^OP5′	siRNA 10	67	−9
H	t^CONHTCGUACGCGGAAUACUUCGATT3′
I	3′ttGCAUGCGCCUUAUGAAGCT^NHCOt^OP5′	siRNA 11	66	−10
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 12	68	−8
L	t^CONHTCGUACGCGGAAUACUUCGAtt3′
I	3′ttGCAUGCGCCUUAUGAAGCT^NHCOt^OP5′	siRNA 13	66	−10
L	t^CONHTCGUACGCGGAAUACUUCGAtt3′
M	3′TTGCAUGCGCCUUAUGAAGCT5′	siRNA 14	70	−6
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 15	68	−8
N	tTCGUACGCGGAAUACUUCGATT3′
M	3′TTGCAUGCGCCUUAUGAAGCT5′	siRNA 16	70	−6
N	tTCGUACGCGGAAUACUUCGATT3′
O	3′ttGCAUGCGCCUUAUGAAGCU5′	siRNA 17	76	—
b	5′CGUACGCGGAAUACUUCGATT3′
a	3′TTGCAUGCGCCUUAUGAAGCU5′	siRNA 18	76	—
P	5′CGUACGCGGAAUACUUCGAtt3′
O	3′ttGCAUGCGCCUUAUGAAGCU5′	siRNA 19	76	—
P	5′CGUACGCGGAAUACUUCGAtt3′