BioMed Research International / 2014 / Article / Fig 3

Research Article

Combined Cytolytic Effects of a Vaccinia Virus Encoding a Single Chain Trimer of MHC-I with a Tax-Epitope and Tax-Specific CTLs on HTLV-I-Infected Cells in a Rat Model

Figure 3

Lack of IFN-γ production was correlated with the resistance of FPM1V.EFGFP/8R cells to killing by 4O1/C8. (a) FPM1V.EFGFP or FPM1V.EFGFP/8R cells (5 × /well) were mixed with 4O1/C8 cells (5 × /well) at an E : T ratio of 10 : 1 in the presence or absence of m8Δ (1 × PFU/well) and subjected to flow-cytometric analysis for the expression of EGFP at the indicated days. Percentage of EGFP positive cells is indicated in each panel. (b) Production of IFN-γ in the supernatants of mixed culture prepared in (a) was measured by ELISA at the indicated days of culture. The data represent the mean ± SD of triplicate wells. Statistical significance was determined as . Similar results were obtained in two independent experiments.