Combined Cytolytic Effects of a Vaccinia Virus Encoding a Single Chain Trimer of MHC-I with a Tax-Epitope and Tax-Specific CTLs on HTLV-I-Infected Cells in a Rat Model
Viability of and viral replication in FPM1V.EFGFP/8R cells exposed to recombinant m8Δs. (a) FPM1V.EFGFP/8R cells were exposed to m8Δ/RT1AlSCTax180L (■), m8Δ/RT1AlSCNLEnv371L (▲), or PBS (●) for 2 hrs at MOI 5. After extensive wash, the cells were cultured for indicated periods and the cell growth was assessed by using cell counting kit 8. The cell viabilities are expressed as percentages of the cell survival of mock-infected cultures. The data are presented as mean ± SD of triplicate wells. (b) FPM1V.EFGFP/8R cells (/well) were exposed to m8Δ at MOI 2, or m8Δ/RT1AlSCTax180L, or m8Δ/RT1AlSCNLEnv371L at MOI 5. After extensive wash, the cells were cultured in the presence or absence of 4O1C8 cells (/well) for indicated periods and were collected for the evaluation of virus proliferation. The proliferation of VVs was determined by titrating the cell lysates. The data are presented as mean ± SD of triplicate wells.