Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6
Establishment of overexpression of Sirt6 in endothelial cells. (a) HUVECs were infected with adenovirus carrying Sirt6 gene (Ad-Sirt6) or empty vector (control, Ad-Con) at different multiplicity of infection (MOI). (a) At 24 hrs after infection, cells were lysed and Sirt6 protein level was determined by Western blot. (b) Cells infected with Ad-Sirt6 were stained with DAPI (blue) for nuclei and anti-Sirt6 antibody (green). Images were taken at 100x magnification.