Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6

Figure 4

Sirt6 overexpression reduces endothelial cell dysfunction induced by H₂O₂. ECs were infected with adenovirus carrying Sirt6 gene (Ad-Sirt6) or empty vector (control, Ad-Con) and treated with 75 𝜇M H₂O₂ or vehicle (H₂O) for 4 days. (a) Cells were subjected to Matrigel-based tube formation assay to determine the angiogenic ability. Images were taken by fluorescence microscopy at 100x magnification and tube length was measured by ImageJ software. (b) eNOS expression in cells was determined by Western blot. ^∗𝑃<0.05 compared with cells infected with Ad-Con and treated with vehicle. ^#𝑃<0.05 compared with cells infected with Ad-Con and treated with H₂O₂.

(a)

(b)