Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6
Sirt6 deletion impairs endothelial cell function. ECs were transfected with Sirt6 siRNA (Sirt6-si) or Control siRNA (Con-si). (a) 10 days after transfection, the angiogenic ability of cells was determined by tube formation assay. Images were taken by fluorescence microscopy at 100x magnification and tube length was measured by ImageJ software. (b) eNOS expression in cells was determined by Western blot. compared with cells transfected with Con-si.