Research Article
Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare Actinomycete Nonomuraea sp. JAJ18
Table 4
Box-Behnken design matrix used in RSM studies along with the experimental and predicted response (antibiotic activity).
| Runs | Variables/coded values | Antibiotic activity (U/mL) ± SEM | Starch | NaCl | MgSO4·7H2O | Experimental | Predicted |
| 1 | − | − | 0 | 123.3 ± 5.78 | 122.94 | 2 | + | − | 0 | 146.6 ± 7.63 | 145.81 | 3 | − | + | 0 | 121.6 ± 12.6 | 122.39 | 4 | + | + | 0 | 106.6 ± 16.1 | 106.96 | 5 | − | 0 | − | 123.3 ± 15.2 | 123.13 | 6 | + | 0 | − | 131.6 ± 17.5 | 131.85 | 7 | − | 0 | + | 145.0 ± 15.0 | 144.75 | 8 | + | 0 | + | 143.3 ± 15.3 | 143.48 | 9 | 0 | − | − | 135.3 ± 15.0 | 135.84 | 10 | 0 | + | − | 113.5 ± 15.3 | 112.89 | 11 | 0 | − | + | 148.6 ± 15.2 | 149.21 | 12 | 0 | + | + | 133.3 ± 5.78 | 132.76 | 13 | 0 | 0 | 0 | 123.3 ± 15.3 | 122.62 | 14 | 0 | 0 | 0 | 123.3 ± 15.3 | 122.62 | 15 | 0 | 0 | 0 | 121.6 ± 17.6 | 122.62 | 16 | 0 | 0 | 0 | 123.3 ± 23.1 | 122.62 | 17 | 0 | 0 | 0 | 121.6 ± 12.6 | 122.62 |
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