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BioMed Research International
Volume 2014, Article ID 906239, 8 pages
http://dx.doi.org/10.1155/2014/906239
Research Article

Anti-Proliferative Effect and Phytochemical Analysis of Cymbopogon citratus Extract

1Department of Biomedical Sciences, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
2Al-Moalim Mohamed Awad Center for Scientific Miracles of Prophetic Medicine, College of Medicine, Taibah University, 3001 Madinah, Saudi Arabia
3Department of Neurosurgery, College of Medicine, Taibah University, 3001 Madinah, Saudi Arabia
4Faculty of Medicine, P.O. Box 30001, Al-Madinah Al-Munawarah, Saudi Arabia

Received 30 December 2013; Revised 15 February 2014; Accepted 21 February 2014; Published 27 March 2014

Academic Editor: Yih-Shou Hsieh

Copyright © 2014 Mohammed F. Halabi and Bassem Y. Sheikh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antiproliferative and antioxidant potential of Cymbopogon citratus (Lemon grass) extracts were investigated. The extracts were isolated by solvent maceration method and thereafter subjected to antiproliferative activity test on five different cancer cells: human colon carcinoma (HCT-116), breast carcinoma (MCF-7 and MDA-MB 231), ovarian carcinoma (SKOV-3 and COAV), and a normal liver cell line (WRL 68). The cell viability was determined using MTT assay. The DPPH radical scavenging assay revealed a concentration dependent trend. A maximum percentage inhibition of 45% and an IC50 of 278?µg/mL were observed when aqueous extract was evaluated. In contrast, 48.3% and IC50 of 258.9?µg/mL were observed when 50% ethanolic extract was evaluated. Both extracts at concentration of 50 to 800?µg/mL showed appreciative metal chelating activity with IC50 value of ?µg/mL to ?µg/mL. Depending on extraction solvent content, extract obtained from 50% ethanolic solvent proved to be more potent on breast cancer MCF-7 cell line (IC50 = 68?µg/mL). On the other hand, 90% ethanolic extract showed a moderate potency on the ovarian cancer (COAV) and MCF-7 cells having an IC50 of 104.6?µg/mL each. These results suggested antiproliferative efficacy of C. citratus ethanolic extract against human cancer cell lines.