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BioMed Research International
Volume 2014, Article ID 913510, 13 pages
http://dx.doi.org/10.1155/2014/913510
Research Article

The Associations of Leishmania major and Leishmania tropica Aspects by Focusing Their Morphological and Molecular Features on Clinical Appearances in Khuzestan Province, Iran

1Parasitology Department, Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Avenue, 1316943551 Tehran, Iran
3Parasitology Department, Medical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

Received 1 July 2014; Revised 19 August 2014; Accepted 27 August 2014; Published 16 September 2014

Academic Editor: Stefano D’Amelio

Copyright © 2014 Adel Spotin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cutaneous leishmaniasis has various phenotypic aspects consisting of polymorphic amastigotes with different genetic ranges. Samples were collected from suspected patients of Khuzestan province. Prepared smears were stained, scaled, and measured using ocular micrometer. The Cyt b, ITS-rDNA, and microsatellite genes of Leishmania were amplified and Leishmania species were identified by molecular analyses. Of 150 examined suspected patients, 102 were identified to Leishmania species (90 L. major, nine L. tropica, and three unidentified). The amastigotes of 90 L. major had regular and different irregular shapes within three clinical lesions with no and/or low genetic diversity. Three haplotypes of Cyt b of L. major were found but no variation was observed using ITS-rDNA gene. Interesting findings were that all nine L. tropica had regular amastigote shapes with more genetic variations, also a patient which had coinfection of L. major, L. tropica, and Crithidia. At least two L. major and L. tropica were identified in suspected patients of the regions. Different irregular amastigotes’ shapes of L. major can be explained by various reservoir hosts and vectors. In contrast, more molecular variations in L. tropica could be justified by genetic characters. Unidentified Leishmania could be mixed pathogens or nonpathogens with mammals’ Leishmania or Crithidia.