The Associations of Leishmania major and Leishmania tropica Aspects by Focusing Their Morphological and Molecular Features on Clinical Appearances in Khuzestan Province, Iran
Figure 5
(a) Evaluating of three amplified PCR bands sensitivity based on provided serial dilution of target DNAs in ITS-rDNA, microsatellite, and Cyt genes. Purified DNA (10 ng) from L. major was used as positive PCR control (+ve PCR). A negative PCR control PCR) without DNA was included in each reaction. Pixel intensity of PCR bands was calculated using Gel Analyser software (2010a) in different genes. (b) To assess the average of different dilutions of PCR bands based on three applied genes in this study, a graph presents the pixel intensity of Cyt b; 110, ITS-rDNA; 82 and ITS2-microsatellite; 90 (from left to right). Bar graph indicates the mean ± SEM.
