The Associations of Leishmania major and Leishmania tropica Aspects by Focusing Their Morphological and Molecular Features on Clinical Appearances in Khuzestan Province, Iran
Table 1
Characteristics of confirmed patients to cutaneous leishmaniasis based on collection study site, personal characters, microscopic observation, and molecular methods.
Province regions (positions)
Collection site
Characteristics
Microscopic observation
Molecular methods
Cities and villages
Sex
Age groups (years)
Lesion duration (month)
Lesion number
Lesion types
Leishmania amastigotes’ positive grading numbers: 1+ to 6+
Leishmania amplification by targeted genes
RFLP with BsuRI & SspI, sequencing and phylogenetic analyses
Female
Male
<1
1–3
3–5
5–10
10–15
15–25
>25
<1
2-3
>3
Single
Double
Multiple
Wet
Dry
Mixed
1+
2+
3+
4+
5+
6+
Total
ITS-rDNA/ & Cyt b & ITS-microsatellite +ve
L. major +ve
L. tropica +ve
Unidentified; Leishmania sp
−ve
+ve
ITS-rDNA
Cyt b
*Mic
−ve
Khuzestan
Ahvaz (central)
Kotabdullah
4
**1
0
1
1
1
2
0
0
2
2
1
3
1
1
4
1
0
0
1
0
2
1
1
0
5
3
1
1
0
2
2
1
Damane kochak
4
6
0
1
2
1
1
4
1
7
1
2
6
2
2
7
2
1
1
1
1
4
2
1
3
10
8
0
2
3
5
0
0
Ashari bozorg
3
7
0
1
1
3
0
3
2
3
4
3
2
5
3
7
2
1
1
1
0
2
2
4
1
10
7
1
0
1
6
0
0
Bahonar
2
2
0
0
0
0
2
1
1
3
1
0
0
0
4
4
0
0
0
0
0
1
1
1
1
4
3
1
0
1
3
1
0
Sheyban
2
3
0
0
0
2
0
2
1
3
2
0
2
2
1
1
4
0
2
1
1
0
1
0
0
5
4
1
0
0
5
0
0
Koye abozar
0
2
1
0
0
1
0
0
0
1
1
0
0
0
2
1
0
1
0
0
0
2
0
0
0
2
1
1
0
0
2
0
0
Kian abad
0
5
0
0
0
2
0
3
0
2
2
1
5
0
0
3
2
0
0
1
1
1
2
0
0
5
3
2
0
0
2
0
0
Shahrake bargh
2
6
0
1
1
2
0
2
2
6
0
2
4
3
1
7
0
1
1
2
1
1
0
3
1
8
3
2
2
1
3
1
0
Padad
2
3
0
0
1
2
0
0
2
4
1
0
4
1
0
2
2
1
0
0
4
1
0
0
0
5
4
1
0
0
3
0
0
Sadeghiyeh
2
0
0
0
0
0
0
0
2
2
0
0
2
0
0
1
0
1
0
0
0
2
0
0
1
2
2
0
0
0
2
0
0
Malashiye
0
3
0
0
0
1
0
2
0
2
1
0
2
1
0
1
2
0
0
1
1
1
0
0
0
3
3
0
0
0
3
0
0
Amaniyeh
1
2
2
1
0
0
0
0
2
2
0
1
2
1
0
2
1
0
0
0
1
2
0
0
0
3
3
1
1
0
2
0
0
400 dastghah
1
3
0
0
2
0
2
0
0
3
0
1
1
3
0
1
3
0
0
0
2
0
2
0
0
4
2
2
0
0
3
0
0
Koye bonovat
1
2
0
0
1
0
0
0
2
2
1
0
2
0
1
1
2
0
0
0
0
3
0
0
1
3
2
1
0
0
2
1
0
Dashteazadegan (western)
Hajiyeh
1
2
0
0
0
1
0
1
1
0
2
1
1
2
0
3
0
0
0
0
1
1
0
1
0
3
2
1
0
0
2
0
1
Soydani
0
4
0
1
0
1
2
0
0
2
1
1
1
1
2
3
1
0
0
0
1
0
1
1
1
4
3
2
0
1
3
1
0
Bostan centre
3
3
0
0
0
0
2
2
2
2
3
1
0
3
3
2
3
1
1
2
1
1
1
0
0
6
5
1
4
0
3
0
0
Shakeriye
2
3
0
0
0
1
2
2
0
2
2
1
2
3
0
3
1
1
0
0
2
1
0
2
0
5
3
2
0
0
4
0
0
Jalaliye
1
2
1
1
0
1
0
0
0
0
1
2
0
2
1
2
1
0
0
1
0
0
0
1
1
3
4
1
1
1
2
1
0
Albo afri
1
3
1
1
2
0
0
0
0
4
0
0
0
1
3
3
0
1
0
0
0
1
2
1
0
4
2
2
0
0
4
0
0
Malekiyeh
0
2
0
0
0
0
1
0
1
0
2
0
1
1
0
1
1
0
0
0
1
0
0
0
1
2
1
0
1
1
2
0
0
Koye abodi
0
2
0
0
0
0
1
1
0
0
2
0
1
0
1
2
0
0
0
0
0
1
0
1
0
2
2
0
0
0
2
0
0
Hoveyze
3
7
1
0
1
1
0
3
4
5
2
3
1
4
5
5
5
0
1
2
2
2
1
2
0
10
3
2
1
0
5
1
1
Farhangian
1
6
0
1
1
1
1
3
0
2
5
0
1
5
1
5
1
1
0
4
1
2
0
0
1
7
3
1
1
1
5
1
0
Ramhormoz (eastern)
Darre khosh
3
6
1
0
0
0
2
5
1
8
1
0
3
2
4
7
2
0
0
1
0
5
1
2
0
9
5
1
2
0
7
0
0
Shardin
1
4
1
1
0
0
0
1
2
0
2
3
2
2
1
3
1
1
0
1
0
1
3
0
0
5
4
1
1
0
3
0
0
Shamilan
3
3
1
2
0
0
1
2
0
3
2
1
1
2
3
5
1
0
0
1
1
1
1
1
1
6
2
1
1
1
5
0
0
Total
43
94
9
12
13
21
19
37
26
66
47
24
51
47
39
86
38
11
7
20
22
45
21
22
13
137
90
30
19
11
90
9
3
137
137
137
137
137
137
150
139
102
= microsatellite, **1 = One patient had coinfections with L. major, L. tropica, and Crithidia.
