Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice

Figure 4

Quantitative analyses of collagen fibers orientation and waviness. Representative image of waviness quantifications of collagen fibers in the tunica adventitia performed using the ImageJ. Photomicrograph at 400 times magnification under polarized light (a). The overall fibers orientation represented as a normalized histogram (b). Waviness analysis showed increased disorientation of collagen fibers in the tunica adventitia of the aortas of mice maintained on HF diet (c). Δ𝐻 is the entropy of the fibers; ^∗∗∗𝑃≤0.001; 𝑛=5 per group.

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