Involvement of p53 Mutation and Mismatch Repair Proteins Dysregulation in NNK-Induced Malignant Transformation of Human Bronchial Epithelial Cells
Figure 2
MMR proteins were downregulated after exposure to NNK. The mRNA (a) and protein (b) levels of MMR genes were detected at 1 week and 8 weeks after NNK exposure by real-time qPCR and Western blot. GAPDH was used as loading control. Values are the means ± SD of at least three independent experiments. . (c) Results of MLH1 and PMS2 MSP assay using primers that amplify methylated (M) or unmethylated (U) alleles specifically. RKO cell was used as the positive control which has a hypermethylated level of MLH1. (d) ChIP assays were performed with indicated antibodies. The precipitated chromatin was quantified by quantitative PCR (qPCR) analysis. Data are presented as means ± SD from three independent experiments. , .
