Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation

Figure 3

Effect of BmooAi (0.6, 1.0, and 1.4 μg) on collagen-induced platelet aggregation. Human PRP was preincubated with the indicated doses of BmooAi for 8 min at 37°C before adding collagen (10 μg/mL). Platelet aggregation was recorded for 10 min in an AggRAM platelet aggregation system with four-channel laser optics (Helena Laboratories, EUA). Results were expressed as an increase in light transmission, where PPP represents the maximum response (100%). Control experiments were performed in the absence of BmooAi.