BioMed Research International / 2014 / Article / Fig 5

Research Article

Multiple Effects of Berberine Derivatives on Colon Cancer Cells

Figure 5

Apoptotic markers. (a) Western blot analysis of PARP-1 cleavage (a), caspase 8 (b) and 3 (c) activation in control cells and in samples treated for 24 h with NAX018 or 100 µM etoposide as a positive control of apoptosis. γ-Tubulin was used as protein loading control. (d) Internucleosomal DNA degradation in HCT116 and SW613-B3 cells untreated (control) and treated with NAX018 or 100 µM etoposide; M: DNA molecular marker. (e) Visualization of mitochondria distribution by immunostaining of the mitochondrial HSP70 protein (red fluorescence) in HCT116 cells untreated (C) and treated for 24 h with 10 µM NAX018. Nuclei were counterstained with Hoechst 33258 (blue fluorescence). Scale bar: 50 µm.

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