BioMed Research International / 2014 / Article / Fig 4

Research Article

Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome

Figure 4

(a) HEK293 cells were transfected with cDNA coding for human MBP with C-terminal FLAG epitope (hMBP-FLAG). After 4 h, the cells were incubated for an additional 20 h with or without GA, as indicated. The cells were then subjected to a cycloheximide chase followed by Western blotting. (b) The HEK293 cells were transfected with cDNAs coding for rhMBP-FLAG along with cDNA coding for the human histone H1.3-FLAG, as indicated. After 24 h, the cells were subjected to a cycloheximide chase followed by western blotting analysis. The percentage of protein remaining was calculated as the ratio of protein at the indicated time-points relative to the initial protein. The data are represented as the mean ± SEM from three separate experiments. denotes .

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