Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome
(a) HEK293 cells were transfected with cDNA coding for human MBP with C-terminal FLAG epitope (hMBP-FLAG). After 4 h, the cells were incubated for an additional 20 h with or without GA, as indicated. The cells were then subjected to a cycloheximide chase followed by Western blotting. (b) The HEK293 cells were transfected with cDNAs coding for rhMBP-FLAG along with cDNA coding for the human histone H1.3-FLAG, as indicated. After 24 h, the cells were subjected to a cycloheximide chase followed by western blotting analysis. The percentage of protein remaining was calculated as the ratio of protein at the indicated time-points relative to the initial protein. The data are represented as the mean ± SEM from three separate experiments. denotes .
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