Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish
Figure 1
Characterization of the zebrafish Tg (−3.1) per1b::luc transgenic line. (a) Bioluminescence in single Tg (−3.1) per1b::luc transgenic embryos during development from 3 hrs postfertilization and under light/dark cycles (12 : 12 at 25°C). Traces of 4 representative animals are shown. Below, white and black horizontal bars indicate the duration of the 12 hours light (white) and 12 hours dark (black) periods of the LD cycles. (b) Mean values of bioluminescence expression profiles from transgenic larvae entrained for 5 days in LD cycles at 25°C and then monitored in constant darkness (DD) and constant temperature (25°C). Below, white, grey, and black horizontal bars represent the 12 hours light, subjective day and dark (or subjective night) periods, respectively. Before transfer to constant darkness, the embryos were previously exposed to 5 LD cycles (5×). (c) Mean of bioluminescence expression profiles from transgenic larvae raised for 8 days in DD conditions (black horizontal bar). (d) Peak (ZT3) and trough (ZT12) values of endogenous rhythmic zfper1b expression in embryos raised under DD (black vertical bars) or LD (grey vertical bars) cycles from 2 to 4 dpf. The expression levels are represented as fold induction values with respect to the lowest value for each set of samples (ZT12 of the second day for both sets). Each time point represents a pool with a minimum of larvae.
