Prolonged Hypocalcemic Effect by Pulmonary Delivery of Calcitonin Loaded Poly(Methyl Vinyl Ether Maleic Acid) Bioadhesive Nanoparticles

Figure 5

A 20% SDS-PAGE slab gel stained with Coomassie showing (1) unstained molecular weight protein markers: 116.0 kDa; β-galactosidase (E. coli), 66.2 kDa; bovine serum albumin (bovine plasma), 45.0 kDa; ovalbumin (chicken egg white), 35.0 kDa; lactate dehydrogenase (porcine muscle), 25.0 kDa; REase Bsp981 (E. coli), 18.4 kDa; 𝛽-lactoglobulin (bovine milk), 14.4 kDa; lysozyme (chicken egg white); (2) 24 h release medium of the optimized sCT nanoparticles (58.1 μg mL⁻¹); (3) blank sample of 24 h release medium of the optimized nanoparticles but without sCT; and (4) standard sample of sCT solution (250 μg mL⁻¹).