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BioMed Research International
Volume 2014, Article ID 932737, 9 pages
Research Article

The Biological Effects of Sex Hormones on Rabbit Articular Chondrocytes from Different Genders

1Department of Biomedical Engineering, I-Shou University, Kaohsiung 81346, Taiwan
2Department of Orthopedics, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung 81300, Taiwan

Received 17 February 2014; Revised 12 May 2014; Accepted 12 May 2014; Published 3 June 2014

Academic Editor: Magali Cucchiarini

Copyright © 2014 Shwu Jen Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to investigate the biological effects of sex hormones (17β-estradiol and testosterone) on rabbit articular chondrocytes from different genders. We cultured primary rabbit articular chondrocytes from both genders with varying concentration of sex hormones. We evaluate cell proliferation and biochemical functions by MTT and GAG assay. The chondrocyte function and phenotypes were analyzed by mRNA level using RT-PCR. Immunocytochemical staining was also used to evaluate the generation of collagen-II. This study demonstrated that 17β-estradiol had greater positive regulation on the biological function and gene expressions of articular chondrocytes than testosterone, with the optimal concentrations of 10−6 and 10−7 M, particularly for female chondrocytes.