The Biological Effects of Sex Hormones on Rabbit Articular Chondrocytes from Different Genders
Figure 5
Immunocytochemical staining in female rabbit chondrocytes following incubation with sex hormones for 3 days. Optic microscopic view of (a) controls, (b) cells incubated with 10−6 M 17β-estradiol, and (c) cells incubated with 10−6 M testosterone. Immunocytochemical detection of Col-II of (d) controls, (e) cells incubated with 10−6 M 17β-estradiol, and (f) cells incubated with 10−6 M testosterone and immunocytochemical staining for combination of Col-I and Col-II in (g) controls, (h) cells incubated with 10−6 M 17β-estradiol, and (i) cells incubated with 10−6 M testosterone. All showed positive immunoreactivity for Col-II, and stronger positive staining was observed in the estradiol group.
