Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides
Figure 1
Schematic representation of the glycosylation reaction catalyzed by G. sylvestre UDP glucose: sterol glucosyltransferase (GsSGT). UDP-glucose dependent GsSGT catalyzed glucosylation of (a) cholesterol to form cholesterol 3-O-β-D-glucoside, (b) ergosterol to form ergosteryl 3-O-β-D-glucoside, and (c) stigmasterol to form stigmasterol 3-O-β-D-glucoside.