Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides
Figure 2
Multiple sequence alignment of protein sequences of microbial and plant SGTs and GsSGT. The SGT accessions used for the alignment are as follows: WsSGTL1 [W. somnifera]: accession number ABC96116.1, 3-β-SGT [A. thaliana]: accession number AEE31976.1, 3-β-SGT [M. truncatula]: accession number XP003592094.1, 3-β-SGT [Z. mays]: accession number ACG25324.1, sterol glucosyltransferase 1 [L. japonicas]: accession number AEX55299.1, UDP-glucose: sterol glucosyltransferase [A. sativa]: accession number CAB06081.1, sterol glucosyltransferase [A. oryzae RIB40]: accession number XP001818661.2, UDP-glucose: sterol glucosyltransferase [C. albicans]: accession number AAD29571.1, UDP-glucose: sterol glucosyltransferase [S. cerevisiae YJM789]: accession number EDN59412.1, 3-β-SGT [A. niger CBS 513.88]: accession number XP001391739.2, UDP-glucose: sterol glucosyltransferase Ugt51D1 [U. maydis]: accession number AAN77910.1, sterol glucosyltransferase [D. discoideum]: accession number AAD28546.1, 3-β-SGT [C. sp. PCC 7507]: accession number AFY35770.1, UDP-glucose: sterol glucosyltransferase [R. baltica SH 1]: accession number NP869730.1, and 3-β-SGT [C. beijerinckii NCIMB 8052]: accession number ABR34421.1. The consensus is represented by conserved amino acid residues and percentage of conservation by a bar graph, respectively. The alignment at the N and C terminal ends showed negligible conservation and is therefore not included in the figure.
