Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides

Figure 3

Phylogenetic analysis of GsSGT with Neighbour-Joining method using MEGA 5.5 software. The protein sequences retrieved from NCBI database included in the analysis comprised plant and microbial SGTs: WsSGTL1 [W. somnifera]: accession number ABC96116.1, 3-β-SGT [A. thaliana]: accession number AEE31976.1, 3-β-SGT [Medicago truncatula]: accession number XP003592094.1, 3-β-SGT [Z. mays]: accession number ACG25324.1, sterol glucosyltransferase 1 [L. japonicas]: accession number AEX55299.1, UDP-glucose: sterol glucosyltransferase [A. sativa]: accession number CAB06081.1, sterol glucosyltransferase [A. oryzae RIB40]: accession number XP001818661.2, UDP-glucose: sterol glucosyltransferase [C. albicans]: accession number AAD29571.1, UDP-glucose: sterol glucosyltransferase [S. cerevisiae YJM789]: accession number EDN59412.1, 3-β-SGT [A. niger CBS 513.88]: accession number XP001391739.2, UDP-glucose: sterol glucosyltransferase Ugt51D1 [U. maydis]: accession number AAN77910.1, sterol glucosyltransferase [D. discoideum]: accession number AAD28546.1, 3-β-SGT [C. sp. PCC 7507]: accession number AFY35770.1, UDP-glucose: sterol glucosyltransferase [R. baltica SH 1]: accession number NP869730.1 and 3-β-SGT [C. beijerinckii NCIMB 8052]: accession number ABR34421.1.