Isocudraxanthone K Induces Growth Inhibition and Apoptosis in Oral Cancer Cells via Hypoxia Inducible Factor-1α
Figure 7
Effects of HIF-1α induction by cobalt chloride (CoCl2) on isocudraxanthone K (IK)-induced HIF-1α expression (a, b), cytotoxicity (c), early (PI−/AV+) and late apoptotic (PI+/AV+) cells (d), and apoptosis-regulated proteins (e, f) in HN4 (a, c, d, e) and HN12 (b, c, d, f) cells. Cells were pretreated with 100 or 200 μM CoCl2 for 6 h and posttreated with 20 μM for 2 days. The histogram shows the quantification of gene expression by densitometry and is presented as fold increases compared to nonstimulated control cells. Statistically significant difference, compared with control, . #Statistically significant difference versus IK, .
