Research Article

Proteomics and Metabolomics for In Situ Monitoring of Wound Healing

Table 1

Potential biomarkers being detected in WF samples of the soft tissue or femoral bone defect.

DescriptionUniProt accessionGene IDDescriptionMW kDa Calc. pIMax. Mascot scoreNumber of unique peptides% sequence coverageReferencesFurther related proteins

Complement FactorsQ6P6G4C1qbComplement C1q subcomponent subunit B26.68.842.1211Cazander et al., 2012 [40]C1qb, C2, C3, C4, C4b, C8g, and C9
G3V615C3Complement C3186.26.52128.38757Cazander et al., 2012 [40]

Serin proteases and inhibitorsQ5RKH1
Q3T1J1
Prtn3
ELANE
Protein Prtn3
elastase 2, neutrophil
27.7
29.5
8.0
9.3
48.9
96.1
2
7
16
28
Eming et al., 2007 [41] 
Buchstein et al., 2009 [42]
A1m, A2m, SERPINA4, SERPINC1, SERPINF2, Ambp, C3, C4, C4b, C8g, Itih1, Itih3, and Itih4

Cytokine receptor antagonistsP20761Il1rnInterleukin-1 receptor antagonist protein20.36.951.5317Ishida et al., 2006 [43]

Matrix metallopeptidasesP08011Mmp8Matrix metallopeptidase 853.26.642.7516 Gutiérrez-Fernández et al., 2007 [44]Anpep, Cpb2, Hpx, and Lta4h
G3V7D0Mmp9Matrix metallopeptidase 978.56.365.648Reiss et al., 2010 [45]

OxidoreductasesD3ZYK8MpoMyeloperoxidase (mapped)51.99.9798.4966Moor et al., 2009 [7]Gpx1, Gpx3, Gsr, HMOX1, Impdh2, Lbr, Ldha, Ldhb, Ldhc, Mdh1, Mdh2, Mpo, Mtco2, Ndufv2, Nos2, Pgd, Prdx1, Prdx2, Prdx6, Sod1, Sod2, Sod3, Tkt, and Txn

S100 proteins and annexinsQ6B345S100a8Protein S100-A810.26.1426.6787Thorey et al., 2001 [38]S100a11, Anxa2, Anxa3, Anxa4, Anxa5, Anxa6, Anxa7, and Anxa11
P50115S100a9Protein S100-A913.17.5375.51584Wyffels et al., 2010 [20]
G3V7W7Anxa1Annexin A138.87.31750.03375Leoni et al., 2013 [46]

Proteins which were reported as biomarker candidates and could be identified in at least 2 samples of the soft tissue or femoral bone defect are grouped according to their annotated protein classes. Additionally, further proteins being identified and belonging to the same protein classes are listed.