Ceftriaxone, a Beta-Lactam Antibiotic, Modulates Apoptosis Pathways and Oxidative Stress in a Rat Model of Neuropathic Pain

Figure 1

Effect of ceftriaxone on the spinal cord protein levels of Bax (21 kDa) and Bcl2 (26 kDa) and relative density of Bax/Bcl2, following western immunoblotting on days 3 (a and b, resp.) and 7 (c and d, resp.) after CCI. Administration of ceftriaxone (Cef 200 mg/kg, i.p.) started when CCI was induced and continued for 7 successive days. The semiquantitative analysis of protein levels was carried out by the “Gel Doc 2000 UV System” (Alliance 4.7). Each lane was loaded with 100 μg of proteins. β-actin is the loading protein control. Data are mean ± SEM (𝑛=3/group). One-way ANOVA followed by Tukey’s post hoc test was used for multiple comparisons. ^#𝑃<0.05 difference between CCI-NS and sham/naive groups. ^∗𝑃<0.05 CCI-Cef versus NS-CCI group (control).

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