Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering
Figure 1
Characterizationof the in vitro binding and release profile of murine anti-BMP-2 mAb-loaded scaffolds. (a) CLSM analysis showing binding of anti-BMP-2 mAb on each scaffold detected by FITC-conjugated goat anti-mouse secondary antibody. Day 1 represents detection of binding of anti-BMP-2 mAb immediately after immobilization of the mAb on the scaffolds, confirming that murine mAb is retained on all tested scaffolds for up to two weeks in vitro. (b) Quantitative analysis of fluorescence intensity showing initial binding (day 1) of anti-BMP-2 mAb to the scaffolds and the in vitro persistence of anti-BMP-2 at 7 and 14 days later (). (c) The in vitro release of anti-BMP-2 mAb was calculated by measuring mAb concentrations in solution at various time points. .
