Prolonged Nitric Oxide Exposure Enhances Anoikis Resistance and Migration through Epithelial-Mesenchymal Transition and Caveolin-1 Upregulation

Figure 1

Effect of nitric oxide donor on cytotoxicity in lung carcinoma H23 cells. (a) Effect of DPTA NONOate on cell viability. Lung cancer H23 cells were treated with various concentrations (0–50 μM) of DPTA NONOate for 24 h. The cell viability was analyzed using the MTT assay. The data are the mean ± S.D. (𝑛=3). ^∗𝑃<0.05 versus the nontreated control. (b) Morphology of H23 cells treated with DETA NONOate for 14 days. (c) Lamellipodia formation in H23 cells treated with NO donor. H23 cells were treated with NO donor at concentrations of 10 and 25 μM for 14 days. The cells were then stained with phalloidin-rhodamine and Hoechst33342 dye.

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