Prolonged Nitric Oxide Exposure Enhances Anoikis Resistance and Migration through Epithelial-Mesenchymal Transition and Caveolin-1 Upregulation

Figure 4

Nitric oxide mediated caveolin-1 upregulation, anoikis resistance and increase migration. (a) Cells were exposed to DETA NONOate for 14 days and Cav-1 level was evaluated by western blotting. (b) Basal level of Cav-1 protein in H23, H292, H460, and A549 lung cancer cells was determined and the immunoblot signals were quantified by densitometry. (c) Anoikis susceptibility of H23, H292, H460, and A549 lung cancer cells was determined by anoikis assay. (d) The relative cell migration of H23, H292, H460, and A549 lung cancer cells was determined. The data are the mean ± S.D. (𝑛=3). ^∗𝑃<0.05 versus the H23 control cells.

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